Coding

Part:BBa_J100116:Design

Designed by: jessica gronniger   Group: Campbell M Lab   (2013-07-17)


AdhE gene cloned into J100112


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 2843
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 2844
    Illegal PstI site found at 2858
    Illegal NotI site found at 2851
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 658
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 2844
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 2844
    Illegal PstI site found at 2858
    Illegal AgeI site found at 743
    Illegal AgeI site found at 1967
    Illegal AgeI site found at 2012
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No internal BsaI sites. The 5' end of Junction D from part BBa_J119301 was changed from TTAA to CGGA. The change was made by iPCR with primers that caused the amplicon to contain the new sequence as shown in part J100116.


Source

All parts taken from Registry.

References